A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei
Identifieur interne : 001065 ( Main/Exploration ); précédent : 001064; suivant : 001066A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei
Auteurs : Heinrich Leonhardt [États-Unis] ; Andrea W. Page [États-Unis] ; Heinz-Ulrich Weier [États-Unis] ; Timothy H. Bestor [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1992.
Abstract
Tissue-specific patterns of methylated deoxycytidine residues in the mammalian genome are preserved by postreplicative methylation of newly synthesized DNA. DNA methyltransferase (MTase) is here shown to associate with replication foci during S phase but to display a diffuse nucleoplasmic distribution in non-S phase cells. Analysis of DNA MTase-β-galactosidase fusion proteins has shown that association with replication foci is mediated by a novel targeting sequence located near the N-terminus of DNA MTase. This sequence has the properties expected of a targeting sequence in that it is not required for enzymatic activity, prevents proper targeting when deleted, and, when fused to β-galactosidase, causes the fusion protein to associate with replication foci in a cell cycle-dependent manner.
Url:
DOI: 10.1016/0092-8674(92)90561-P
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei</title><author><name sortKey="Leonhardt, Heinrich" sort="Leonhardt, Heinrich" uniqKey="Leonhardt H" first="Heinrich" last="Leonhardt">Heinrich Leonhardt</name></author><author><name sortKey="Page, Andrea W" sort="Page, Andrea W" uniqKey="Page A" first="Andrea W." last="Page">Andrea W. Page</name></author><author><name sortKey="Weier, Heinz Ulrich" sort="Weier, Heinz Ulrich" uniqKey="Weier H" first="Heinz-Ulrich" last="Weier">Heinz-Ulrich Weier</name></author><author><name sortKey="Bestor, Timothy H" sort="Bestor, Timothy H" uniqKey="Bestor T" first="Timothy H." last="Bestor">Timothy H. Bestor</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7B4D6F4778CC2B19337E11FAB42FB3AC4B360518</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1016/0092-8674(92)90561-P</idno><idno type="url">https://api.istex.fr/document/7B4D6F4778CC2B19337E11FAB42FB3AC4B360518/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000B95</idno><idno type="wicri:Area/Main/Curation">000B80</idno><idno type="wicri:Area/Main/Exploration">001065</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Exploration">001065</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei</title><author><name sortKey="Leonhardt, Heinrich" sort="Leonhardt, Heinrich" uniqKey="Leonhardt H" first="Heinrich" last="Leonhardt">Heinrich Leonhardt</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Anatomy and Cellular Biology Harvard Medical School Boston, Massachusetts 02115</wicri:regionArea><wicri:noRegion>Massachusetts 02115</wicri:noRegion></affiliation></author><author><name sortKey="Page, Andrea W" sort="Page, Andrea W" uniqKey="Page A" first="Andrea W." last="Page">Andrea W. Page</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Anatomy and Cellular Biology Harvard Medical School Boston, Massachusetts 02115</wicri:regionArea><wicri:noRegion>Massachusetts 02115</wicri:noRegion></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Massachusetts</region></placeName><wicri:cityArea>† Present address: Department of Biology, Massachusetts Institute of Technology, Cambridge</wicri:cityArea></affiliation></author><author><name sortKey="Weier, Heinz Ulrich" sort="Weier, Heinz Ulrich" uniqKey="Weier H" first="Heinz-Ulrich" last="Weier">Heinz-Ulrich Weier</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Laboratory Medicine Division of Molecular Cytometry University of California at San Francisco San Francisco, California 94143</wicri:regionArea><wicri:noRegion>California 94143</wicri:noRegion></affiliation></author><author><name sortKey="Bestor, Timothy H" sort="Bestor, Timothy H" uniqKey="Bestor T" first="Timothy H." last="Bestor">Timothy H. Bestor</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Anatomy and Cellular Biology Harvard Medical School Boston, Massachusetts 02115</wicri:regionArea><wicri:noRegion>Massachusetts 02115</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">71</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="865">865</biblScope><biblScope unit="page" to="873">873</biblScope></imprint><idno type="ISSN">0092-8674</idno></series><idno type="istex">7B4D6F4778CC2B19337E11FAB42FB3AC4B360518</idno><idno type="DOI">10.1016/0092-8674(92)90561-P</idno><idno type="PII">0092-8674(92)90561-P</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tissue-specific patterns of methylated deoxycytidine residues in the mammalian genome are preserved by postreplicative methylation of newly synthesized DNA. DNA methyltransferase (MTase) is here shown to associate with replication foci during S phase but to display a diffuse nucleoplasmic distribution in non-S phase cells. Analysis of DNA MTase-β-galactosidase fusion proteins has shown that association with replication foci is mediated by a novel targeting sequence located near the N-terminus of DNA MTase. This sequence has the properties expected of a targeting sequence in that it is not required for enzymatic activity, prevents proper targeting when deleted, and, when fused to β-galactosidase, causes the fusion protein to associate with replication foci in a cell cycle-dependent manner.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><country name="États-Unis"><noRegion><name sortKey="Leonhardt, Heinrich" sort="Leonhardt, Heinrich" uniqKey="Leonhardt H" first="Heinrich" last="Leonhardt">Heinrich Leonhardt</name></noRegion><name sortKey="Bestor, Timothy H" sort="Bestor, Timothy H" uniqKey="Bestor T" first="Timothy H." last="Bestor">Timothy H. Bestor</name><name sortKey="Page, Andrea W" sort="Page, Andrea W" uniqKey="Page A" first="Andrea W." last="Page">Andrea W. Page</name><name sortKey="Page, Andrea W" sort="Page, Andrea W" uniqKey="Page A" first="Andrea W." last="Page">Andrea W. Page</name><name sortKey="Weier, Heinz Ulrich" sort="Weier, Heinz Ulrich" uniqKey="Weier H" first="Heinz-Ulrich" last="Weier">Heinz-Ulrich Weier</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/SchutzV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001065 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001065 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= SchutzV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:7B4D6F4778CC2B19337E11FAB42FB3AC4B360518
|texte= A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei
}}
| This area was generated with Dilib version V0.6.38. |  |